Formation of precipitate in AIE conjugated antibody is normal. Make sure the precipitate is thoroughly resuspended before use either by (1) Vortexing for 10 seconds or (2) Soaking the antibody in ultrasonic bath for 30 seconds.
Suggested dilution for Western Blot: 1:1000-1:5000
Western blot for the detection of beta LH subunit at different concentration (from left to right, 220 ng to 22 ng). Intact LH protein was loaded and ran into the SDS PAGE gel. After the protein transfer from gel to the PVDF membrane, the membrane was washed with TBST and block with 5% BSA. The membrane was then incubated with mouse monoclonal primary antibody against beta-subunit for LH (1:1000) in TBST with 5% BSA. The AIE Green conjugated Goat anti-Mouse IgG antibody (1/1000X) in TBST with 5% BSA was used to detect the primary antibody on the PVDF membrane. Image was captured by ChemiDoc using Stain Free Blot(Green) and Alexa 647(Blue) channels.